Heterologous Complementation in Yeast Reveals the Solute Specificity of a Nucleobase Cation Symporter 1 (NCS1) from Nicotiana tabacum
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منابع مشابه
Heterologous complementation studies reveal the solute transport profiles of a two-member nucleobase cation symporter 1 (NCS1) family in Physcomitrella patens.
As part of an evolution-function analysis, two nucleobase cation symporter 1 (NCS1) from the moss Physcomitrella patens (PpNCS1A and PpNCS1B) are examined--the first such analysis of nucleobase transporters from early land plants. The solute specificity profiles for the moss NCS1 were determined through heterologous expression, growth and radiolabeled uptake experiments in NCS1-deficient Saccha...
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The single cell alga Chlamydomonas reinhardtii is capable of importing purines as nitrogen sources. An analysis of the annotated C. reinhardtii genome reveals at least three distinct gene families encoding for known nucleobase transporters. In this study the solute transport and binding properties for the lone C. reinhardtii nucleobase cation symporter 1 (CrNCS1) are determined through heterolo...
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Nucleobases and their precursors are vital organic molecules in al living cels due to their role in nucleic acid biochemistry, sugar metabolism, as wel as serving as energy molecules. In plants, the salvage and catabolism of nucleobases requires an intricate membrane transport mechanism that ensures their movement between cels and also within cels between the organeles and cytoplasm. One of sev...
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Miller, Sara E. M.S., Purdue University, August 2012. Functional Characterization of Two Putative Nucleobase Transporters in Arabidopsis Using Heterologous Complementation in Yeast. Major Professor: George S. Mourad. To identify the substrate profile function for AtNAT5, one of the twelve members of the NAT/NCS2 gene family putatively identified to transport xanthine and/or uric acid, and to id...
متن کاملبیان فراوان ژن Δ1 – پرولین- 5-کربوکسیلات سنتتاز( p5cs ) ، با هدف افزایش مقاومت به تنش‌های اسموتیک در گیاه تراریخت توتون ( Nicotiana tabacum cv. Xanthi )
Proline as a key osmoregulating solute in plants plays an overriding role in osmotic pressure adjustment of the cell under water stress conditions. In plant, a bifunctional enzyme delta-1-pyrroline-5-carboxylate synthetase (p5cs) promotes and directs proline synthesis during drought stress conditions. The activity of this enzyme is strongly induced to increase proline concentration within the c...
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تاریخ انتشار 2013